Autosampler

Column Care and Practice

• Majors, Ronald E. "The Care and Feeding of Modern HPLC Columns." LC GC. 16(10), 1998, 900-909.

Compound Information

Gas Chromatography

Alltech. "Technical Tips: Split Injections: What? When? Why? and How?" Bulletin #430; p 10. {file}

General Biochemistry and Medical Chemistry

• BioMedNet
• Links to journals, databases, and other information of interest to chemists, biologists, and medical people
• Must sign up for a free membership

General Chemistry

• ChemWeb.com
• Links to journals, databases, and other chemistry information
• Must sign up for a free membership
• Martindale's Virtual Chemistry Desk
• Links to many useful chemistry sites on the Internet.

General Chromatography

General Spectroscopy

• Spectroscopy page.
• Contains links to other spectroscopy sites and indices to the journal Spectroscopy.

HPLC Operation

Liquid Crystals in Chromatography

Modeling

• Al-Haj, Mehdi Ahmed; Kaliszan, Roman; Nasal, Antoni. "Test Analytes for Studies of the Molecular mechanism of Chromatographic Separations by Quantitative Structure-Retention Relationship." Anal. Chem. 71, 1999, 2976-2985.
• Kaliszan, Roman. Quantitative Structure-Retention Relationships. Anal. Chem. 64(11), 1992, 619A-631A.
• NIH Modeling Pages.
• Thomas, Edward V. A Primer on Multivariate Calibration. Anal. Chem. 66(15), 1994, 795A-804A.

Measuring Column Efficiency

Nomenclature

• IUPAC Organic Nomenclature Page

On-line Abstract Services

• Medline
• A free, on-line abstract searching service that primarily serves the medical community but also abstracts many chemistry journals including Analytical Chemistry and other journals of separations and spectroscopy.

Perfluorinated Stationary Phases

• Brindle, R.; Albert, K. Stationary phases with chemically bonded fluorine ligands: A new approach for environmental analysis of pi-electron containing solutes. J. Chrom. A . 1997, 757, 3-20.
• deMiguel, I.; Exbrayat, S.; Samain, D. Study of the Selective Retention of Fluorinated Compounds on Perfluorinated Stationary Phases. Chromatographia. 1987, 24, 849-853.
• Ecknig, W.; Trung, B.; Radeglia, R. Group Separation of Alkyl-Substituted Aromatic Hydrocarbons by High-Performance Liquid Chromatography Using Perfluorocarbon Modified Silica Gel. Chromatographia. 1982, 16, 178-182.
• Felix, G.; Meiouet, F. A New Way to Graft Silica Gel: Hydride Silanes. J. Chrom. Sci. 1991, 29, 457-459.
• Haky, J. et al. Perfluorooctyl and perfluorobutyl bonded alumina stationary phases for high performance liquid chromatography. J. Liq. Chrom. & Rel. Technol. 1996, 19 (2), 307-332.
• Haas, A.; Kohler, J. Fluorinated Ligands in HPLC. Chromatographia.1981, 14 (6), 341-344.
• Hendershot, S.; Koharski, D.; McNamara, P. Use of fluorine-containing stationary phases for the separation of an alkyl bromide from its hydrocarbon analog by high-performance liquid chromatography. J. Chrom. A. 1997, 762, 159-65.
• Hirayama, C. et al. Immobilization of Highly-Oriented Perfluoroalkyl Polymers onto Porous Silica Gels for High Performance Liquid Chromatography. Polymer Journal. 1994, 26 (4), 499-503.
• Hirayama, C. et al. Isocratic HPLC Separation of Aromatic Hydrocarbons and Fluorine-Containing Compounds Using Perfluoroalkyl Polymer Packings. Chromatographia. 1992, 33 (9/10), 473-477.
• Hirayama, C. et al. Totally Organic Stationary Phase from Perfluoroalkyl Acrylate for Liquid Chromatography. J. Chrom. 1989, 465, 241-248.
• Jinno, K.; Nakamura, H. Retention Characteristics of Fluorinated Bonded Silica Phase in Reversed-Phase Liquid Chromatography. Chromatographia. 1994, 39 (5/6), 285-293.
• Pesek, J.; Matyska, M.; Hemphala, H. HPLC Evaluation of Mono-ol, Butylphenyl and Perfluorinated Columns Prepared via Olefin Hydrosilation on a Silica Hydride Intermediate. Chromatographia. 1996, 43 (1/2), 10-17.
• Sadek, P.; Carr, P. A Study of Solute Retention in Reversed-Phase HPLC on Hydrocarbonaceous and Three Fluorinated Bonded Phases. J. Chrom. 1984, 288, 25-41.

Silica

• Kirkland, J.J. The Use of Porous Silica-Based Column Packings in HPLC Method Development. Current Issues in HPLC Technology (Supplement to LC GC) May 1997, 1997, S46-S55.

Statistical Tests

Rorabacher, D.B. Anal. Chem. 1991.

Syringes

Temperature Control - HPLC

• Wolcott, Robert G.; Dolan, John W. "Column Temperature Effects in Gradient Elution." LCGC. 1998, 16, 1080-1083.

Tests

Theses and Papers

Toxicology Information

• Prudent Practices in the Laboratory: Handling and Disposal of Chemicals.  A document from the National Academy of Sciences which discusses good chemical disposal practices.
• http://www.hhmi.org/science/labsafe/lcss/ - a link within the above document on the Howard Hughes Medical Institute (HHMI) Web site which allows you to search by chemical name for chemical properties and hazards.
• Toxnet (Toxicology Data Network) from the National Library of Medicine
• Location to get information on risks of compounds

Writing in Science

• What's Right about Science Writing
• Article from The Scientist that addresses briefly addresses many points about scientific writing